Bio-Rad Comparative Proteomics Kit I: Protein Profiler Module - Manual (Page 3)

11. Heat fish samples and actin and myosin
standard to 95°C for 2–5 min.
12. Load your gel:
Lane Volume Sample
1 & 2 empty empty
3 5 µl Precision Plus Protein Kaleidoscope
prestained standards (Stds)
4 10 µl fish sample 1
5 10 µl fish sample 2
6 10 µl fish sample 3
7 10 µl fish sample 4
8 10 µl fish sample 5
9 10 µl actin and myosin standard
(AM)
10 empty empty
13. Electrophorese for 30 minutes at 200 V in 1x
TGS electrophoresis buffer.
14. After electrophoresis, remove gel from cassette
and transfer gel to a container with 25 ml Bio-Safe
Coomassie blue stain per gel and stain gel for
1 hour, with gentle shaking for best results.
Lesson 3 Quick Guide
1. Discard stain and destain gels in a large
volume of water for at least 30 minutes to overnight,
changing the water at least once. Blue-stained
bands will be visible on a clear gel after destaining.
2. Dry gels using GelAir cellophane.
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