Bio-Rad Genes in a Bottle™ Kit - Manual (Page 2)

8. Place the cap on your tube, and gently invert it a few
times.
9. Place your tube in a test tube rack or beaker in the
water bath and incubate at 50°C for 10 minutes.
Remove your tubes from the water bath.
10. Obtain the tube of cold alcohol from your instructor or
at the common workstation. Holding your tube at a
45° angle, fill your tube with cold alcohol, by adding
approximately 10 mls to your tube. It will take repeated
additions to add 10 ml of the cold alcohol using the
disposable plastic transfer pipet.
11. Place your cap on your tube, and let it sit undisturbed
for 5 minutes. Write down anything you observe
happening in the tube.
12. After 5 minutes, slowly invert the tube 5 times to help
the DNA, which has begun to precipitate, to aggegate.
13. With a disposable plastic transfer pipet, carefully
transfer the precipitated DNA along with approximately
750 µl to 1 ml of the alcohol solution into a small glass
vial provided in the DNA necklace kit (166-2200EDU).
If you are not going to make a DNA necklace, save
your DNA in a flip-top tube provided in this kit.
12
Water bath
50°C for 10 min