Bio-Rad Microbial Culturing Module - Manual (Page 5)

Kit Inventory Checklist
Kit Components Number/kit (
✔)
Ampicillin 2 vials
❐
LB nutrient agar powder (to make 500 ml) 1 pouch
❐
Petri dishes, sterile, bag of 20 2 bags
❐
Culture tubes 60 mm, sterile, bag of 25 3 bags
❐
Inoculation loops, sterile, 10 µl, pack of 10 loops 6 pks
❐
E. coli HB101 K-12, lyophilized 1 vial
❐
LB broth capsules, bag of 12 (to make 50 ml each) 1 bag
❐
Disposable plastic transfer pipets (DPTPs), pack of 10 1 pack
❐
Refills Available Separately
Microbial Culturing kit refill package (Catalog #166-5021EDU), contains ampicillin, LB broth
capsules, LB agar and
E. coli strain HB101 K-12
LB nutrient agar powder (Catalog #166-0600EDU) 1 pkg
Ampicillin (Catalog #166-0407EDU) 1 vial
E. coli strain HB101 K-12 (Catalog #166-0408EDU) 1 vial
Recommended Accessories
Microwave, autoclave, or heated magnetic stir plate
Glass flasks
Graduated cylinder
Thermometer
Incubation oven
Safety Issues
Ampicillin may cause allergic reactions or irritation to the eyes, respiratory system and skin. In
case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing. Ampicillin is a member of the penicillin family of antibiotics.
Those with allergies to penicillin or any other member of the penicillin family of antibiotics
should avoid contact with ampicillin.
The host organism in this kit, an E. coli K-12 strain, is not pathogenic. However, handling of
E. coli K-12 requires the use of Standard Microbiological Practices. These practices include but
are not limited to the following. Work surfaces are decontaminated once a day after any spill of
viable material. All contaminated liquid or solid wastes are decontaminated before disposal.
Persons wash their hands: (i) after they handle materials involving organisms containing
recombinant DNA molecules, and (ii) before exiting the laboratory. All procedures are performed
carefully to minimize the creation of aerosols. Mechanical pipetting devices are used; mouth
pipetting is prohibited. Eating, drinking, smoking, and applying cosmetics are not permitted in
the work area. Wearing protective eyewear and gloves is strongly recommended.
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