Bio-Rad ProteinChip Fractionation and Purification Products - Manual

4. Centrifuge the spin column at ~80 x g (1,000 rpm)*
for 30 seconds to remove the buffer.
5. Replace the bottom cap of the spin column.
6. Add 200 µl 50 mM Tris buffer, pH 9 to the column,
then replace the top cap on the column.
7. Vortex the column to mix the sorbent with
the buffer.
8. Remove the top and bottom caps of the spin
column; set the column upright in a new 1.5 ml
microcentrifuge tube.
9. Centrifuge the spin column for 30 seconds to
remove the buffer.
10. Repeat steps 5–9 twice, for a total of three
buffer washes.
11. Replace the bottom cap on the spin column.
The sample can now be added to the spin column.
12. If the column is not used right away, add 0.5 ml
buffer, pH 9 to prevent dehydration.
* Recommended speeds for an Eppendorf 5417R centrifuge.
© 2006 Bio-Rad Laboratories, Inc.
Eppendorf is a trademark of Eppendorf-Netheler-Hinz GmbH. HyperD is
a trademark of Pall Corporation.
The SELDI process is covered by US patents 5,719,060, 5,894,063,
6,020,208, 6,027,942, 6,124,137, 6,225,047, 6,528,320, 6,579,719,
and 6,734,022. Additional US and foreign patents are pending.
ProteinChip ® Q
Spin Columns
Instruction Manual
Catalog #C54-00017
For technical support,
call your local Bio-Rad office, or
in the US, call 1-800-4BIORAD
(1-800-424-6723).
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Ordering Information
Catalog # Description
C54-00017 ProteinChip Q Spin Columns, 20
C57-30080 ProteinChip Q10 Arrays, A-H format, 12