Bio-Rad DC™ Protein Assay - Manual (Page 4)

Vortex mixer
Plastic or glass cuvettes with 1 cm path length matched to laboratory spec-
trophotometer
Test tube rack to hold 13 x 100 mm test tubes
For microplate assay:
Microtiter plates
Reservoir for working reagent
Pipets for reagent preparation
Pipets accurately delivering 5 µl, 25 µl, and 200 µl
Microplate reader set to 750 nm
3.1 Safety Considerations
Eye protection and gloves should be worn while using this product. Consult
MSDS at the end of this manual for additional information.
Section 4
Reagent Compatibility
The listed compounds were tested and found to be compatible with the Bio-
Rad DC Protein Assay. In some cases, the presence of one or more of these
substances will effect a change in the response of the protein to the assay
reagents; therefore, the standard should always be prepared in the same buffer
as the sample.
10% SDS 1% CHAPS 2% NP-40
1% Triton † X-100 1% CHAPSO 1% Thesit †
1% Tween † 20 1% Octyl glucoside 1% Brij † -35
0.2% C
12
E
8
* 0.1 M Tris, pH 8 0.5 M NaOH
0.5 M HCl 0.5 M (NH
4
)
2
SO
4
0.025 M EDTA
0.05 M CaCl
2
0.4 M Guanidine HCl 4 M Urea
0.05% Sodium azide 1 mM DTT (dithiothreitol)
Note: The DC Protein Assay is incompatible with 2-mercaptoethanol (BME)
† BRIJ and TWEEN are registered trademarks of Atlas Chemical. THESIT is
a registered trademark of Desitin Arzneimittel GMBH. TRITON is a registered
trademark of Rohm and Haas.
*octaethyleneglycol dodecyl ether
2
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