Bio-Rad Protein Assay - Manual (Page 5)

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1.2 Product Description
Protein Assay Dye Reagent Concentrate (catalog number 500-0006)
contains 450 ml of solution containing dye, phosphoric acid, and
methanol. One bottle of dye reagent concentrate is sufficient for 450
assays using the standard assay procedure, or 2,250 assays using the
microassay procedure.
The Dye Reagent Concentrate can be purchased in a kit with one of
two standards: Bovine gamma globulin (Kit I, catalog number 500-0001)
or bovine serum albumin (Kit II, catalog number 500-0002).
The Bio-Rad Protein Assay is for research use only.
1.3 Materials Required but Not Supplied
For standard assay
Spectrophotometer set to 595 nm
Cuvettes with 1 cm path length matched to laboratory spectropho-
tometer. Bio-Rad’s disposable polystyrene cuvettes (catalog number
223-9950) are recommended
13 x 100 mm test tubes
Test tube rack for 13 x 100 mm test tubes
Vortex mixer
Whatman #1 filter (or equivalent) and funnel for dye reagent prepara-
tion
Graduated cylinders, pipets, and containers for reagent preparation
and storage
Pipets accurately delivering 100 µl and 5.0 ml
For microplate assay
Microtiter plates
Pipets accurately delivering 200 µl and 800 µl
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