Bio-Rad MicroRotofor™ Cell User Manual
Page 9
Fig. 7. Placing the focusing assembly
Fig. 8. Fitting the cathode assembly into
onto the anode of the chassis.
the cathode of the chassis.
3. Using a 10 ml syringe, add electrolyte solutions to the electrode
assemblies (Figure 9).
(Note: The electrode membrane storage solution from the starter kit can be
used. Alternatively fresh electrolyte solutions can be prepared)
I.
Add 6 ml 0.1 M H
3
PO
4
through the vent hole of the anode assembly
(red).
II.
Add 6 ml 0.1 M NaOH through the vent hole of the cathode
assembly (black).
Fig. 9. Adding electrolyte to the anode assembly.
4. Close the cooling block cover and tighten the screw.
5. Place the lid on the chassis.
6. Attach the power cord to the back of the MicroRotofor chassis and
connect it to an electrical outlet.
7. Connect the MicroRotofor cell to a vacuum source. We recommend
installation of a vacuum trap between the cell and the vacuum system.
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