Bio-Rad ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) - Manual (Page 6)

- Wheaton Potter-Elvehjem tissue grinder (for example,
VWR catalog #62400-700)
- Kontes tight-fitting microcentrifuge-pestle combination
(for example, VWR catalog #KT749520-0000)
• 1X Phosphate buffered saline (for cultured cells)
• Protease inhibitor(s)
• Reducing agent (for example, DTT, TBP, or TCEP)
• Carrier ampholytes (for example, Bio-Lyte

3/10
ampholyte)
• RC DC™ Protein Assay (Bio-Rad catalog #500-0121 or
#500-0122)
Section 3
Storage Conditions
The unopened kit can be stored at room temperature.
CPEB and PSB diluent should be stored at 2–8°C after
opening. Use aseptic technique when handling CPEB and
PSB diluent to prevent contamination. For storage of the
ReadyPrep 2-D cleanup kit, follow the instructions provided
in the kit.
3
4110147A5.qxd 1/15/2003 2:49 PM Page 6