Bio-Rad ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) - Manual (Page 11)

11. Make an appropriate dilution of the nuclear protein
fraction into complete PSB or other strongly chaotropic
2-D rehydration/sample buffer before performing
IEF/2-D gel electrophoresis. Refer to Section 5.1 for
guidelines on selecting the appropriate volume of
complete PSB or 2-D rehydration/sample buffer and
Section 5.2 for directions on preparing PSB and other
strongly chaotropic 2-D rehydration/sample buffers.
12. Process an appropriate amount of the cytoplasmic
protein fraction through the ReadyPrep 2-D cleanup
kit. To determine an appropriate amount, refer to the
guidelines in Section 5.1. Follow the ReadyPrep 2-D
cleanup kit protocol provided. At the end of the 2-D
cleanup procedure, resuspend the cytoplasmic protein
pellet in the appropriate amount of complete PSB or
other 2-D rehydration/sample buffer and perform
IEF/2-D analysis.
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