Bio-Rad ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) - Manual (Page 5)

Section 2
Kit Specifications
Each ReadyPrep protein extraction kit (cytoplasmic/nuclear)
provides sufficient reagents to perform up to 50 extractions
of 50 mg of cells or tissue. The procedure can easily be
scaled up to accommodate larger amounts of cells or
tissue.
Items Supplied With Kit
One bottle containing 50 ml of cytoplasmic protein
extraction buffer (CPEB)
One bottle containing 25 g of protein solubilization
buffer (PSB)
One bottle containing 30 ml of PSB diluent
One ReadyPrep 2-D cleanup kit
Items Required But Not Provided
• 1.5 ml microcentrifuge tubes
• Microcentrifuge capable of spinning at 12-16,000 x g
at 4°C.
• Vortex mixer
• Homogenizer/tissue grinder.
- 20 gauge syringe needle (for cultured cells)
- Wheaton Dounce tissue homogenizer (for example,
VWR catalog #62400-595)
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