Bio-Rad ReadyPrep Sequential Extraction Kit - Manual (Page 5)

Section 3. Storage
Store the lyophilized contents of the kit, Reagents 1, 2, and 3,
unopened at room temperature.
Store the sealed ampoule of reducing agent TBP unopened
at room temperature.
Section 4. Reagent Preparation
Reagent 1: Add 50 ml of deionized water to the vial of
Reagent 1. Swirl the vial gently at intervals until the contents
are completely dissolved. Reconstituted Reagent 1 consists
of 50 ml of 40 mM Tris base per vial.
Reagent 2: Add 6 ml of deionized water to each vial of
Reagent 2. Swirl the vials gently at intervals until the contents
are completely dissolved. It will take some time for the
contents of the vial to dissolve. The vial will chill as the urea in
the solids dissolves. Rehydrated Reagent 2 consists of 10 ml
of 8 M urea, 4% (w/v) CHAPS, 40 mM Tris, and 0.2% (w/v)
Bio-Lyte 3/10 per vial.
Reagent 3: Add 6.3 ml of deionized water to each vial of
Reagent 3. Swirl the vials gently at intervals until the contents
are completely dissolved. It will take some time for the
contents of the vials to dissolve. The vial will be chilled as the
urea and thiourea in the solids dissolve. The SB 3-10
detergent will not dissolve completely until the solution has
been warmed slightly. It is often sufficient to roll a vial of
Reagent 3 between the hands for a short period of time to
dissolve the SB 3-10. In some situations, it might be
3
4006167b.qxd 08/28/2000 6:43 PM Page 3