Bio-Rad ReadyPrep Sequential Extraction Kit - Manual (Page 12)

10
Cell Sample
Supernatant 1
Supernatant 2
Supernatant 3
Insoluble Pellet from
Extraction 2
Insoluble Pellet from
Extraction 1
Insoluble Pellet from
Extraction 3
Reagent 1
Solution 2
Solution 2
Gel 1
Gel 2
Gel 3 Solution 3
Step 1
Step 2
Step 3
An example of the sequential extraction of an E. coli
preparation is shown in the figure.
Schematic Illustration of the 3-Step Sequential
Extraction Protocol
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