Bio-Rad MicroRotofor™ Cell Lysis Kits - Manual (Page 4)

Each MicroRotofor run using 2.5 mg total protein yields ten
150–250 µl fractions, and the protein distribution among
the fractions will vary depending on the sample. For example,
using spinach leaf extracts and ampholytes spanning the
pH range 3–10, fractions 3–5 typically contain the highest
amounts of protein.
Certificates of analysis and MSDS forms are available upon
request.
Items Supplied With Kit
Protein solubilization buffer (PSB) (contains urea, 25 g
thiourea, NDSB 201, and Tris)
PSB diluent (contains CHAPS and Tris) 30 ml
ReadyPrep 2-D cleanup kit 1 kit
Instruction manual 1
Items Required But Not Provided
• 1.5 ml microcentrifuge tubes
• 14 ml disposable culture tubes with closures (VWR
catalog #60818-725)
• Microcentrifuge capable of spinning at 20,000 x g
• Sonicator with probe
2
10005508A.qxp 8/11/2006 11:50 AM Page 2