Bio-Rad MicroRotofor™ Cell Lysis Kits - Manual (Page 3)

Section 1
Introduction
MicroRotofor lysis kits provide convenient, efficient methods
for the preparation of protein samples for fractionation with
the MicroRotofor cell. The MicroRotofor lysis kit (plant) is
designed for use with soft plant tissues and plant cell cultures,
and employs tissue maceration and/or solubilization into a
chaotropic extraction buffer (Vuillard et al. 1995). For added
convenience, the extraction buffer is also used as the
sample buffer for isoelectric focusing (IEF) either with the
MicroRotofor cell or with IPG strips. The kit also includes
components of the ReadyPrep
™ 2-D cleanup kit, which are
used to remove pigments and other interfering molecules
from the plant samples.
Section 2
Kit Specifications
Each MicroRotofor lysis kit (plant) provides sufficient reagent
to perform at least 10 extractions (from 1 g samples) and to
prepare sample for 10 MicroRotofor runs. More than 10
extractions will be possible with the kit if the samples are
smaller than 1 g or if the sample is applied onto IPG strips
and not prefractionated with the MicroRotofor cell. Using
this kit, extraction from 1 g spinach leaf tissue yields
~20 mg total protein.
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