Bio-Rad MicroRotofor™ Cell Lysis Kits - Manual (Page 12)

Table 3. Recommended protein loads for IPG
strips.
IIP PG G S Sttrriip p L Leen ng gtth h
7 7 ccm m 1 11 1 ccm m 1 17 7 ccm m 1 18 8 ccm m 2 24 4 ccm m
Rehydration volume/strip 125 µl 185 µl 300 µl 315 µl 410 µl
Protein load
Silver stain 5–20 µg 20–50 µg 50–80 µg 50–80 µg 80–150 µg
Coomassie G-250 50–100 µg 100–200 µg 200–400 µg 200–400 µg 400–800 µg
Flamingo ™ ,
SYPRO Ruby 2.5–75 µg 10–150 µg 25–300 µg 25–300 µg 40–600 µg
The suggestions made in Table 3 are a general rule of
thumb. Increased protein loads may be required for
micro-range IPG strips and for samples of higher protein
complexity.
Section 6
Reference
Vuillard L et al., Non-detergent sulfobetaines: a new class of mild
solubilization agents for protein purification, Biochem J 305, 337–343 (1995)
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