Bio-Rad MicroRotofor™ Cell Lysis Kits - Manual (Page 6)

Section 4
Instructions for Use
Preparation of Protein Solubilization Buffer (PSB)
Solution
1. Use only freshly rehydrated buffer. Discard any unused
buffer.
2. Allow the PSB dry reagent to warm to room
temperature before opening the bottle. Shake the PSB
dry reagent bottle for 10–15 sec. Weigh an appropriate
amount (each gram of dry reagent will prepare
approximately 2 ml buffer solution). Use 1 ml of PSB
per 60 µl of wet cell pellet (Table 1).
Table 1. Guideline for PSB preparation.
# Samples Volume PSB PSB Dry PSB Diluent Approximate
(60 µl wet cell Needed (ml) Reagent (g) (ml) Volume PSB
pellet) Prepared (ml)
1 1 1 1.1 2
2 2 2 2.2 4
3 3 2 2.2 4
4 4 3 3.3 6
5 5 3 3.3 6
3. For each gram of dry reagent, add 1.1 ml of PSB
diluent.
4. Vortex periodically and incubate at room temperature
until you have a clear solution (2–3 min).
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