Bio-Rad MicroRotofor™ Cell Lysis Kits User Manual
Page 5
• ReadyPrep
™
proteomic grade water (catalog #163-2091)
or other ultrapure water
•
β-mercaptoethanol
Items Recommended But Not Required
• Protease inhibitor (for example, Sigma catalog #P8215)
• ReadyPrep reduction-alkylation kit (catalog #163-2090)
• ReadyPrep 2-D cleanup kit (catalog #163-2130)
Section 3
Storage Conditions
Shipped at ambient temperature. Store kit components as
individually marked. Note: Lyticase is shipped at room
temperature, but should be stored at –20°C upon receipt.
This kit has a warranty period of 1 year from shipment
date, assuming all components are stored as indicated on
each label.
Component
Store at
Protein solubilization buffer (PSB), 25 g
RT
PSB diluent, 30 ml
4°C
Yeast suspension buffer, 15 ml
RT
Lyticase, 1.5 U/µl, 2 x 0.5 ml
–20°C
3
10005509A.qxp 8/11/2006 11:30 AM Page 3