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Bio-Rad MicroRotofor™ Cell Lysis Kits User Manual

Page 5

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• ReadyPrep

proteomic grade water (catalog #163-2091)

or other ultrapure water

β-mercaptoethanol

Items Recommended But Not Required

• Protease inhibitor (for example, Sigma catalog #P8215)
• ReadyPrep reduction-alkylation kit (catalog #163-2090)
• ReadyPrep 2-D cleanup kit (catalog #163-2130)

Section 3
Storage Conditions

Shipped at ambient temperature. Store kit components as
individually marked. Note: Lyticase is shipped at room
temperature, but should be stored at –20°C upon receipt.
This kit has a warranty period of 1 year from shipment
date, assuming all components are stored as indicated on
each label.

Component

Store at

Protein solubilization buffer (PSB), 25 g

RT

PSB diluent, 30 ml

4°C

Yeast suspension buffer, 15 ml

RT

Lyticase, 1.5 U/µl, 2 x 0.5 ml

–20°C

3

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