Bio-Rad MicroRotofor™ Cell Lysis Kits - Manual (Page 4)

the fractions will vary depending on the sample. For example,
using Saccharomyces cerevisiae and ampholytes spanning
the pH range 3–10, fractions 2–4 typically contain the most
protein.
Certificates of analysis and MSDS forms are available upon
request.
Items Supplied With the Kit
Protein solubilization buffer (PSB) (contains urea, 25 g
thiourea, NDSB 201, and Tris)
PSB diluent (contains CHAPS and Tris) 30 ml
Yeast suspension buffer (contains sodium phosphate, 15 ml
sodium chloride, potassium chloride, and
potassium phosphate)
Lyticase enzyme, 5 units/µl, prepared from Arthrobacter 2 x 0.5 ml
luteus, MW = 54.6 kD, pI = 6.33 Swiss-Prot/TrEMBL
accession number E13B-ARTSW (Q59146). See Appendix for
more information
Instruction manual 1
Items Required But Not Provided
• 1.5 ml microcentrifuge tubes
• Microcentrifuge capable of spinning at 20,000 x g
• Sonicator with probe
• DTT reducing agent (catalog #161-0611) or TBP reducing
agent (catalog #163-2101)
• Carrier ampholytes
• RC DC
™ protein assay (catalog #500-0121 or 500-0122)
• Glycerol
2
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