Bio-Rad Quantum Prep Plasmid Kits - Manual (Page 3)

Section 1
Introduction
1.1 Overview
The Quantum Prep plasmid midiprep kit has been
optimized for the rapid purification of DNA from 40 to
50 ml of liquid culture. This kit uses the optimized
alkaline lysis protocol and patented DNA binding matrix
found in the Quantum Prep plasmid miniprep Kit.
1 This
matrix, made up of the silicon dioxide exoskeleton of
diatoms, results in DNA of the highest purity and yield
with the minimum of processing steps. A single midiprep
can be processed in approximately 45 minutes and requires
no ethanol precipitation. The Quantum Prep kit was
optimized using high-copy number plasmids grown in
either Luria Broth or Terrific Broth.
2 It requires only 40 ml
of culture and a single harvesting step. Multiple samples
can be processed simultaneously with minimal increase
in time. DNA purified using the Quantum Prep midiprep
kit can be used directly for enzymatic modification, cell
transfection and transformation, fluorescent sequencing
and
in vitro transcription. Each lot is qualified for
automated fluorescent sequencing, yield, and enzymatic
manipulation. Yields of high copy plasmid are in the range
of 100 µg to over 300 µg from 40 ml of culture.
1
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