Bio-Rad Aurum™ Vacuum Manifold - Manual (Page 7)

Section 9
Troubleshooting Guide
Problem Possible Cause Possible Solution
Insufficient negative Open vacuum regulator Close vacuum regulator
pressure in vacuum by turning completely
manifold clockwise
Manifold top not seated Ensure uniform contact
properly on base between manifold top
and base
Purification plate or CAP Press down gently on
not fully seated the corners of the plate
or CAP to seal
Unused CAP luer fittings Close unused luer
not closed completely fittings completely with
caps
Gasket residue preventing Rinse manifold with
even contact between
distilled water
manifold parts
Lower gasket misaligned Press gasket back into
groove
Physical damage to Replace vacuum
manifold or CAP (e.g., manifold or CAP
chips and cracks)
Purge step in purification If closure of wells or
protocol columns (e.g., with
sealing tape) restores
negative pressure, no
corrective action
needed
Excessive noise Purification plate or CAP Press down gently on
during vacuuming not fully seated plate or CAP to seat
Gasket residue preventing Rinse manifold with
even contact between
distilled water
manifold parts
Worn manifold gasket Replace gasket
Problem Possible Cause Possible Solution
Incorrect negative Vacuum source Connect vacuum
pressure from connected to left to right side of vacuum
vacuum regulator side of vacuum
Clogging of Insufficient negative See “Insufficient
purification plate pressure in vacuum negative pressure
or CAP columns manifold in vacuum manifold”
problem above
9 8