Bio-Rad Chromatography Standards - Manual (Page 6)

Fig. 1.
a. Bio-Sil SEC 125 column (exclusion limit*
≈
80,000).
b. Bio-Sil SEC 250 column (exclusion limit
≈
300,000).
c. Bio-Sil SEC 400 column (exclusion limit ≈ 800,000).
Peaks:
A. Protein aggregates (void peak)
B. Thyroglobulin
C.
γ
-globulin
D. Ovalbumin
E. Myoglobin
F. Vitamin B
12
Conditions
Buffer 0.05 M Na
2
HPO
4
, 0.05 M NaH
2
PO
4
,
0.15 M NaCl, pH 6.8
Column 300 x 7.8 mm
Flow rate 1.0 ml/min
Sample 20 µl
Chart speed 1 cm/min
Detection UV @ 280 nm; 1.0 AUFS
*Nominal exclusion limits given for globular proteins.
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