Bio-Rad UNO® Monolith Cation Exchange Columns - Manual (Page 3)

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Fig. 1. Drawing of UNO column and UNO replacement column.
The columns are supplied in a storage buffer of 0.1 M NaCl + 20% ethanol. The counter
ion for the Q column is Cl - and for the S column is Na + . Prior to initial use and after extend-
ed storage periods, each column should be conditioned as described below (steps 1–4). Always
use HPLC grade reagents and filter and degas the buffers. During this operation do not exceed
the following flow rates:
S-1 or Q-1 1 ml/min
S-6 or Q-6 2 ml/min
S-12 or Q-12 3 ml/min
1. Wash with 5 column volumes of water. Elevated backpressures may occur when wash-
ing with deionized water. Do not exceed 700 psi.
2. Wash with 5 column volumes of low ionic strength start buffer [ e.g. 20 mM Tris-HCl
(Q) or 20 mM Sodium Phosphate(S)].
3. Wash with 5 column volumes of high ionic strength elution buffer (e.g. starting buffer +
1.0 M NaCl).
4. Wash with 5 column volumes of low ionic strength equilibration buffer [e.g. 20 mM
Tris-HCl (Q) or 20 mM Sodium Phosphate(S)].
The column may now be further equilibrated in start buffer at the desired flow rate.
Tubing, .020 I.D. x 15 cm (1) Cap (11)
Shipping tube (12)
O-ring (13)
Plug (14)
Centering ring (15)
Glass column (16)
Centering ring (15)
Plug (14)
O-ring (13)
Cap (11)
Tube end fitting, 1/4 x 28 (2)
End cap (3)
Shield (4)
Bed support (5)
O-ring (6)
Spacer (7)
Distribution screen (8)
Frit (9)
Glass tube (10)
Frit (9)
Distribution screen (8)
Spacer (7)
O-ring (6)
Bed support (5)
End cap (3)
Tube end fitting, 1/4 x 28 (2)
Tubing, .020 I.D. x 15 cm (1)
UNO Column UNO Replacement Column