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Labnet FastPette Pro - Ultimate Pipette Controller User Manual

Page 7

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4

Filling the pipette

Before aspirating is started, set the speed by successive pressing the SPEED switch (fig. 1C)
until the display shows the right speed (fig. 1E).

• HIGH speed - fast aspirating (fig. 2.1)

• LOW speed - slow aspirating (fig. 2.2)

It is recommended to choose the LOW position for pipettes with a volume up to 5 ml, and
the HIGH position for pipettes with a volume greater than 5 ml. Holding the pipette con-
troller in such a way that the pipette is in vertical position immerse the pipette end in the liq-
uid to be drawn up (fig. 3B), and press the aspiration button gently. The pipette filling speed
depends on how deep the aspirating button has been pressed. The deeper the button
is pressed the faster the liquid is aspirated into the pipette.

It is recommended to draw a slightly greater liquid volume than required (due to meniscus
above the required volume mark), adjusting the aspiration speed, particularly in the final filling
stage, so as not to overfill the pipette.

Setting the volume

After aspirating the liquid to the pipette, in order to remove the solution rests from the out-
side surface of the pipette, its end shall be dried with absorbent paper that does not leave
impurities. Then set the required liquid volume precisely. Pressing the dispense button gently
(fig. 3C), dispense the excessive liquid from the pipette until the meniscus of the liquid
aligns exactly with the required volume mark on the pipette.

Emptying the pipette

Holding the vessel in inclined position, place the pipette end in contact with the vessel wall
and press the dispense button gently (fig. 3C). The dispensing intensity may be adjusted
depending on how deep the dispense button has been pressed. The deeper the button
is pressed the faster the outflow of liquid from the pipette.

The FASTPETTE PRO has two dispense modes. The dispense mode is selected by succes-
sive pressing the MODE switch (fig. 1D) until the display shows the right mode (fig. 1E).

• dispense in gravity mode marked with the „drop” icon on the display (fig. 2.4) - the dis-

pense is effected in gravity mode, which means that the liquid flows out of the pipette by
its own weight. The outflow speed is adjusted depending on how deep the dispense but-
ton has been pressed.

• forced dispense with a blow marked with the „arrow” icon on the display (fig. 2.5) – the dis-

pense is effected in gravity mode, however, when the dispense button is pressed to the
middle position, the pump is started and fast emptying of the pipette with a blow is effected.

CAUTION:
During gravimetrical dispensing the pipette is not completely emptied due to the characte -
ristics of pipettes used with the pipette controller.

FASTPETTE PRO INSTRUKCJA (474P.) Nr rys. 015.9001.00.07_FASTPETTE PRO INSTRUKCJA (474P.) 11-11-30 16:58