C.B.S. Scientific DCX-900C - Manual (Page 14)

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DCX-900C Large Format Instructions 9-30-13
SECTION 4
Running Conditions
4.1 Recommended Power for Slab Gels:
Precise electrophoresis conditions will vary according to the number and type of
gels used, buffer conditions employed, power input, and the general goal of the
experiment. Refer to reference section 4.2 for in depth discussions on practical and
theoretical approaches to protein gel electrophoresis.
4.1.1 SDS PAGE Gels
Run Voltage Starting Current Ending Voltage Approx. Run TIme
90VDC 30mA/gel CC 110 volts/gel 4-5 hours
4.1.2 General Recommendations
If running only one gel, keep the volts the same but reduce the mA’s by half. Keep in
mind that as the thickness of gel increases, the mA’s increase proportionally.
At constant voltage, the proteins will migrate at a constant rate during electropho-
resis with adequate heating appropriate for denaturing gels. Increasing the voltage/
mA (for a single gel thickness and percentage) will speed mobility but increase the
risk of overheating.
If using cooling base the power input and the migration rate can be increased. The
joule heating generated by the higher power is offset by the cooling effect of the
buffer between the gels.
4.2 References
1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical
Approach. 3 rd edn. Oxford University Press, Oxford. Ch. 1,3.
2. Sambrook, J., Fritsch, Russell, D. (2001). Molecular Cloning. A Laboratory
Manual. 3 rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor,
New York. A8.40-A8.55
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith,
J.A., Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 2,
Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 10.