C.B.S. Scientific SGU-2640T-02 - Manual (Page 9)

C.B.S. œ Scientific Submarine/Horizontal 9
B. Gel Casting in Gel Tray.
1. Tape both ends of gel tray with tape.
2. Position the gel comb(s) in the gel tray.
Using a pipette or a pipetor, run a bead of
agarose solution along the edges of the gel
tray where it meets the tape to assure a
leak-proof seal. Then pour the agarose
solution into the gel tray. Allow the agarose
slab to cool uncovered until it turns opaque.
NOTE: (Optional storage) Pre-cast gels can
be stored for up to one month if wrapped in
plastic wrap and stored at 4
°C. Remove
combs prior to storing.
3. Remove gel tape at each end. Position the
tray in the unit, remembering, current travels
from negative (black cathode) towards
positive (red anode).
3.3 Running the Gel
1. Add enough buffer to fill both reservoirs and overflow the surface of the gel to a depth
of 2-3mm. Gently remove comb(s). Flush out any air bubbles in the wells. Load the
samples into the sample wells.