C.B.S. Scientific SGU-2640T-02 - Manual (Page 8)

C.B.S. œ Scientific Submarine/Horizontal 8
3.2 Gel Casting
The Submarine/Horizontal gel unit provides the user with two options for gel casting: (a) gel
casting directly into unit using gel tray or (b) gel casting in gel tray outside of unit.
A. Casting Directly into Unit Using Gel Tray.
1. Place gel tray onto raised bed of unit. Insert the tapered baffles simultaneously on either side
of gel tray and slide them into the grooves next to the bed, handles to the outside. The tapered
baffles will rest friction tight against the gel tray.
2. Place gel comb(s)into desired slot(s)
provided on gel tray. Remember, current
travels from negative (black cathode)
towards positive (red anode). Pour the
agarose solution onto the gel bed. Allow the
agarose slab to cool uncovered until it turns
opaque. Agarose around combs will take a
few more minutes to cool than other part of
the slab.
3. Slide the baffles out of their slots and store
in the slots provided at either end of the unit
near both terminals.