C.B.S. Scientific SGU-2640T-02 - Manual (Page 7)

C.B.S. œ Scientific Submarine/Horizontal 7
Instructions for Use
3.1 Submarine/Horizontal Unit Preparation
Place Submarine/Horizontal unit on a level work surface in an authorized work area. Level the
unit by placing the bubble level in the center of the unit and adjusting the four leveling screws on
the of base until the unit is level.
Place the adjustable height comb(s) assembly in the gel tray. Loosen the white screws holding
the comb to the backing and adjust comb depth (generally 2mm). Gently retighten the adjusting
screws and set the comb aside. See Table 1 and 2 for comb volumes.
Coolant Circulation:
A selection of white tubing adapters (right angled and straight) is provided with each unit. For
coolant circulation, screw tubing adapters into fittings on side of internal cooling chamber, located
under the gel bed. Connect a heavy wall tubing which will not kink (if tubing kinks, excessive
pressure build-up may damage unit and create coolant leaks) and attach a regulated pump or
recirculating water bath (follow manufacturer’s instructions). Flow rate is not to exceed 3 liters
per minute. Do NOT use tap or house water as it can be subject to large fluctuations in pressure.
Buffer Recirculation:
Buffer recirculation is recommended for longer runs to maintain uniform pH and ionic strength
and to reduce buffer changes. Unit is shipped with buffer circulation ports sealed with four white
plugs. For cycling buffer, remove plugs and replace with threaded tubing adapters. Using tubing
adapters and pump, cycle buffer from anode (+) to cathode (-).
RIGHT ANGLE
TUBE FITTINGS
FLOW
PUMP HEAD
PERISTALTIC
STRAIGHT
TUBE FITTINGS
- +
TO CHILLER