C.B.S. Scientific MGU-602T User Manual
Page 7
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Tel# 858-755-4959
6
SECTION 3
Instructions for Use
3.1 Midi-Gel Unit Preparation and Gel Casting
(illustrated steps below show removable lid, but flip lid steps are very similar)
Place Midi-Gel unit on a level work surface in an authorized work area. Place the adjustable
height comb assembly in the gel tray. Loosen the white screws holding the comb to the backing
and adjust comb
depth (generally 1- 2mm). Gently tighten the adjusting screws and set the comb aside.
See Table 1 for comb loading volumes.
The Midi-Gel unit provides the user with two options for gel casting: (a) gel casting directly into
unit using gel tray or (b) gel casting in gel tray outside of unit.
A. Casting Directly into Unit Using Gel Tray.
1. Place gel tray onto raised bed of unit. Insert
the tapered baffles, simultaneously on either
side of gel tray and slide into the grooves
next to the bed, handles to the outside.
Press down firmly on the tapered baffles.
The tapered baffles will rest friction tight
against the gel tray.
2. Position the gel comb(s). Remembering,
current travels from negative (black cathode)
towards positive (red anode). Pour the
agarose solution onto the gel bed. Allow the
agarose slab to cool uncovered until it turns
opaque. Agarose around combs will take a
few more minutes to cool than other parts of
the slab unless using Teflon coated
aluminum combs which cool faster.
3. Slide the baffles out of their slots and store
in the slots provided at either end of the unit
near both terminals.