C.B.S. Scientific MGU-602T - Manual (Page 11)

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Ethidium Bromide Staining
EtBr can be premixed with buffers and agarose for use during electrophoresis.
Add to agarose only after temperature has fallen below 55°C. Gels can also be stained
after electrophoresis in a soaking tray. Use EtBr at a final concentration of 0.1µg/ml
from a 1mg/ml stock solution. Ethidium Bromide is a powerful mutagen. ALWAYS
wear gloves, eye protection and protective clothing. Dispose of solutions in accordance
with the safety regulations of your institution.
4.3 References
1. Hames, B.D., Rickwoood, D. (ed.) (1990). Gel Electrophoresis of Nucleic Acids.
A Practical Approach. 2
nd edn. IRL Press, Oxford. Ch. 2.
2. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning. A Laboratory
Manual. 2
nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New
York. Ch 6.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A.,
Struhl, K. (ed) (1993). Current Protocols in Molecular Biology. Vol. 1, Greene
Publishing Associates, Inc. and John Wiley & Sons, Inc., Ch. 2.
SECTION 5
Maintenance of Equipment
5.1 Care and Handling
The plastic components of the Midi-Horizontal units are fabricated from acrylic and polycarbonate.
Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass.
As with any laboratory instrument, adequate care ensures consistent and reliable performance.
After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a
soft cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed
gently with water and a non-abrasive detergent, and rinsed and dried as above. Never use
abrasive cleaners, glass cleaning sprays or scouring pads to clean the components, as these will
damage the unit and components.
Additional precautions:
•
Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated
hydrocarbon solvents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at 37°C.
A brief rinse with DEPC-water is sufficient after a thorough wash.
5.2 Maintenance
The following inspection and maintenance procedures will help maintain the safety and
reliable performance of the Midi-Horizontal systems. Replacement parts can be ordered by call
1-858- 755-4959 or by contacting your local distributor.
• Banana plugs and power cords should be inspected regularly. If the banana plugs become
loose or do not feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of wear or
damage (e.g. cracks, nicks, abrasions, or melted insulation), replace them immediately.
• The platinum wire is secured to the banana jack by compression between a stainless washer
and the jack nut. The nut/washer interface should be tight and free of corrosion.