C.B.S. Scientific EBU-222 - Manual (Page 10)

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SECTION 5
MAINTENANCE OF EQUIPMENT
5.1 CARE AND HANDLING
The plastic components of the Electrophoretic Blotting units are fabricated from acrylic and polycarbonate.
Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any
laboratory instrument, adequate care ensures consistent and reliable performance.
After each use, rinse buffer chamber, cassettes and Scotch Brite pads with de-ionized water. Wipe dry with a
soft cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed gently with
water and a non-abrasive detergent, and rinsed and dried as above. Never use abrasive cleaners, glass cleaning
sprays or scouring pads to clean the components, as these will damage the unit and components.
Additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon sol-
vents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at 37°C. A brief rinse
with DEPC-water is sufficient after a thorough wash, followed by a quick rinse in 50% ethanol.
• Electrostatic charge and heat affix the conductive platinum surface covering the titanium. It can be
scratched off or damaged by using sharp objects or scouring pads. This will disturb the continuity of the
electric field and have a negative effect on transfers.
• The stainless electrode can be permanently damaged by corrosion if the polarity between anode and cath-
ode is reversed. CAUTION: Be certain of power connections before initiating transfer.
5.2 MAINTENANCE
The following inspection and maintenance procedures will help maintain the safety and reliable performance of
the Blotting systems. Replacement parts can be ordered by calling 1-858-755- 4959 or by contacting your local
distributor.
• Banana plugs and power cords should be inspected regularly. If the banana plugs become loose or do not
feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of wear or damage (e.g.
cracks, nicks, abrasions, or melted insulation), replace them immediately.
• The platinum wire is secured to the banana jack by compression between a stainless washer and the jack
nut. The nut/washer interface should be tight and free of corrosion.