C.B.S. Scientific EBU-102P - Manual (Page 5)

C.B.S. œ Scientific
5
Electro-Blotting System
SECTION 1
General Information
1.1
Introduction
Electroblotting is a faster, easier, and more efficient method to permanently transfer PAGE gels
to nitrocellulose and nucleic acids to nylon, than capillary (passive diffusion) blotting. For a
similar transfer, traditional “capillary blotting” can take 6-24 hours to complete compared to only 2
hours or less using electroblotting. The C.B.S. Scientific Electrophoretic Blotting Systems come
equipped with; two electrode panels, one color-coded gel cassette assembly, electrophoresis
tank with cooling chamber and safety cover with attached power leads. The EBU-102 or EBU-
102P gel cassette size is 15cm x 21.5cm and can transfer up to three gels at one time. The
EBU-202 gel cassette size is 9 x 11cm and can transfer up to 4 gels at one time.
1.2 Specifications
Constructions:
Buffer chamber, safety cover Acrylic
Electrode panels Acrylic and platinum wire .012" diameter
Power cords FR Urethane rated 7500VDC @ 200mA,
65
°C
Gel cassette Perforated fiberglass panels red and
black, two Scotch-Brite® pads, and one
sponge pad
Safety Certification EN61010-1-1993 (IEC1010-1)
EBU-102 or
EBU-102P
EBU-202
Shipping
Weight 7 lbs. 5 lbs.
Overall Size
(l)x(w)x(h) cm 19 x 11 x 28.5 14 x 13 x 16
Maximum Gel Size - cm 15.3 x 21.5 10 x 10
Distance between
electrodes -cm
8.7 10
Recommended buffer
volume
1 cassette
2 cassettes
3 cassettes
4 cassettes
3.75 liters
3.76 liters
3.77 liters
N/A
1.1 liter
0.9 liter
0.8 liter
0.7 liter
1.3 Safety
Power to the Electro-Blotter is to be supplied by an external DC voltage power supply that must
be ground isolated so that the DC voltage output floats with respect to ground. For any power
supply used, the maximum specified operating parameters for the units are:
Maximum Limits
100 VDC
150 watts power
1500mA current
50
°C ambient temperature
Current to the unit, provided from the external power supply, must enter the unit through the lid
assembly, providing a safety interlock to the user. Current to the unit is broken when the lid is
removed. Do not attempt to use the unit without the safety lid, and always turn the power
supply off before removing the lid, or when working with the unit in any way. Follow
safety precautions specified by the power supply manufacturer.