C.B.S. Scientific EBU-102P - Manual (Page 14)

C.B.S. œ Scientific
14
Electro-Blotting System
SECTION 5
Maintenance of Equipment
5.1
Care and Handling
The plastic components of the Electrophoretic Blotting units are fabricated from acrylic and
polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and
chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and
reliable performance.
After each use, rinse buffer chamber, cassettes and Scotch Brite pads with de-ionized water.
Wipe dry with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all
components may be washed gently with water and a non-abrasive detergent, and rinsed and
dried as above. Never use abrasive cleaners, glass cleaning sprays or scouring pads to clean
the components, as these will damage the unit and components.
Additional
precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene,
halogenated hydrocarbon solvents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended
periods at 37
°C. A brief rinse with DEPC-water is sufficient after a thorough
wash, followed by a quick rinse in 50% ethanol.
• Electrostatic charge and heat affix the conductive platinum surface covering the
titanium. It can be scratched off or damaged by using sharp objects or scouring
pads. This will disturb the continuity of the electric field and have a negative
effect on transfers.
• The stainless electrode can be permanently damaged by corrosion if the polarity
between anode and cathode is reversed. CAUTION: Be certain of power
connections before initiating transfer.
5.2 Maintenance
The following inspection and maintenance procedures will help maintain the safety and reliable
performance of the Blotting systems. Replacement parts can be ordered by calling 1-858-755-
4959 or by contacting your local distributor.
• Banana plugs and power cords should be inspected regularly. If the banana
plugs become loose or do not feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of
wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace
them immediately.
• The platinum wire is secured to the banana jack by compression between a
stainless washer and the jack nut. The nut/washer interface should be tight and
free of corrosion.