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C.B.S. Scientific EBU-102P User Manual

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C.B.S. œ Scientific

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Electro-Blotting System

4.2 References

Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (ed.)

(1993). Current Protocols in Molecular Biology. Vol. 2, Greene Publishing Associates,
Inc. and John Wiley & Sons, Inc., Ch.10.


Burnette, W.N. (1981). Western blotting: Electrophoretic transfer of proteins from sodium dodecyl

sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with
antibody and radioiodinated protein A. Anal. Biochem. 112:195-203.

Peluso, R.W. and Rosenberg, G.H. (1987). Quantitative electrotransfer of proteins from sodium

dodecyl sulfate polyacrylamide gels onto positively charged nylon membranes. Anal.
Biochem.
162:389-398.

Perides, G. Plagens, U., and Traub, P. (1986). Protein transfer from fixed, stained and dried

polyacrylamide gels and immunoblot with protein A-gold. Anal. Biochem. 152:94-99.

Tesfaigzi, J., Smith-Harrison, W., and Carlson, D.M. (1994). A simple method for reusing

western blots on PVDF membranes. Biotechniques. 17:268-269.

Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of proteins from

polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc.
Natl. Acad. Sci. U.S.A.
76: 4350-4354.

Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. 2

nd

ed.

Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 18.47-18.61.

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