C.B.S. Scientific EBU-204 - Manual (Page 9)

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5 Assemble blotting stack as shown in Figure 3 With cassette wide open assemble
components on black side in the following order: buffer saturated sponge pad, gel
equilibrated in transfer buffer, buffer saturated transfer membrane, then buffer saturated
blotting paper. Smooth with gloved finger or roll with glass rod to be sure no bubbles exist
between the gel and the transfer membrane
6. Insert blotting cassettes into the lower reservoir. Red side of cassette
should face toward red electrode panel.
red side
high molecular weight
bands at this end
blotting paper
gel
foam pad
black side
membrane
Figure 1 Figure 2
Figure 3