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C.B.S. Scientific EBU-204 User Manual

Page 13

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13

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SECTION 5

Maintenance of Equipment

5.1 Care and Handling

The plastic components of the Electrophoretic Blotting units are fabricated from cell

cast acrylic and polycarbonate Electrodes and connectors are made from pure

platinum, stainless steel, and chrome plated brass As with any laboratory instrument,

adequate care ensures consistent and reliable performance

After each use, rinse buffer chamber, cassettes and sponge pads with de-ionized water.

Wipe dry with a soft cloth or paper towel, or allow to air dry Whenever necessary, all

components may be washed gently with water and a non-abrasive detergent, and

rinsed and dried as above Never use abrasive cleaners, glass cleaning sprays or

scouring pads to clean the components, as these will damage the unit and components

Additional precautions:

·

Do not autoclave or dry-heat sterilize the apparatus or components.

·

Do not expose the apparatus or components to phenol, acetone,

benzene, halogenated hydrocarbon solvents or undiluted alcohols.

·

Avoid prolonged exposure of the apparatus or components to UV

light

·

Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for

extended periods at 37°C. A brief rinse with DEPC-water is sufficient

after a thorough wash, followed by a quick rinse in 70% ethanol

·

Electrostatic charge and heat affix the conductive platinum surface

covering the titanium It can be scratched off or damaged by using

sharp objects or scouring pads This will disturb the continuity of the

electric field and have a negative effect on transfers.

·

The stainless electrode can be permanently damaged by corrosion

if the polarity between anode and cathode is reversed. CAUTION:

Be certain of power connections before initiating transfer