Eppendorf Thermoblock slides - Manual (Page 5)

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To mount the exchangeable Thermoblock "slides", open the lid by
simultaneously pressing the two pressure points on the front edge of the lid. The
pressure points are labeled with "push". Adjust the exchangeable Thermoblock
on the basic device in such a way that the label can be seen from the front.
Screw it tight using an Allen key.
After loading the exchangeable Thermoblock with up to 4 slides, and prior to
temperature control / rotation, the lid must be closed by simultaneously pressing
the two pressure points. It is absolutely necessary to ascertain that the lid has
been correctly closed prior to starting the device. The slides are only fixed when
the lid is closed, and only then rotation can begin.
After placing the slides thermoblock on Thermomixer comfort / R basic devices,
the block is identified. This automatically limits the maximum rotation speed to
1400 rpm. If the block is not identified automatically by older Thermomixer
comfort basic devices, it is vital that you observe the maximum rotation speed of
1400 rpm. Please contact our Customer Service department (see page 22) to
update the software in your basic device.
Four slide positions are provided on the exchangeable Thermoblock. A reaction
area must be positioned above the microarray on the slide surface in order to
carry out the reaction. Commercially available hybridization chambers or cover
glass of various sizes are currently used to create the reaction chamber. When
using hybridization chambers or cover glass it is important to consider that 4 mm
of the front sides of a standard slide are not available as reaction area due to the
fixing by the lid of the exchangeable Thermoblock. The total height of the slide
and the hybridization chamber shall also not exceed 5 mm. Also it is important
that the hybridization chamber doesn’t overlap the edges of the slide.
The hybridization chamber or the cover glass must be fixed on the slide surface
and sealed as air-tight as possible, thus preventing the leakage of reaction
solution during temperature control and / or rotation.
3 Mounting
4 Application
3 Mounting / 4 Application
ThermoblSlides.fm Seite 14 Donnerstag, 5. Juli 2007 12:05 12