Eppendorf Heat Sealer - Manual (Page 8)

8
After installation, follow the procedures set out below:
– Check that the unit is connected to an appropriate power supply and leave for
20 – 25 minutes to settle down to correct operating temperature. The green power lamp
should be illuminated and the amber heater lamp flicker on and off.
– Slide the plate to be sealed on to the platform of the sealer, making sure that it is flat
between the two centralizing springs.
– Deep well plates can be sealed without a base plate. If the plate to be sealed does not have
its own support skirt to keep it perfectly flat, place it in an appropriate base plate (PCR
Plate 96 should be placed in the metal 96-well base plate supplied with this instrument.
PCR Plate 384 has an alternative base plate (see recommended accessories, ordering
information).
– Once the heater plate has reached operating temperature, the amber heater light on the
side of the sealer will go off.
– Place the sealing film over the plate, ensuring that the sealing surface is face down.
– Holding the handle on the top of the unit, push the heater down to the plate. Once the
heater is resting on top of the plate, continue to push down to compress the floating springs
which are located behind the plate. Once these are fully compressed (a distance of only
5 mm), this applies a force equivalent to a weight of 10 kg to the surface of the plate.
– Hold in this position for approximately 2 – 5 seconds (this time will vary significantly
depending on the plate material, the surface area and the sealing film being used. An initial
time/seal study is strongly recommended for each new type of plate) then turn the plate by
180° and hold for another 2 – 5 seconds (again, an initial time/seal study is strongly
recommended).
The heater plate is set to approximately 170 °C. Sealed plates can also reach
temperatures of over 50 °C very quickly. There is a risk of burns!
Coming into contact with heater plate and sample plates should be avoided
until temperatures of around 30 °C or less are reached.
– Return the heater to the top of the track.
– Remove the plate.
Note: The instrument is fitted with an internal heater with a maximum temperature of approxi-
mately 170 °C when ON. Take care to avoid skin contact with the heater plate and to
prevent contact between the heater plate and any material which may be damaged or
ignited.
Foil-backed film may remain hot for a number of seconds after sealing; care should be
taken in handling these plates.
4.1 Sealing
Operation
4 Operation
4
HeatSealer_en.fm Seite 8 Montag, 13. März 2006 1:41 13