Techne Prime Elite Satellite - Manual (Page 40)

40
GRADIENT
The gradient feature of the Prime Elite Satellite can be useful in optimising the annealing conditions for
primers. A gradient can be set around a temperature in any step of a program. The set temperature is
the temperature in the middle columns of the block and the gradient is the variation at the two
extremes of the block; the left hand columns being the coolest and the right hand columns the hottest.
The maximum gradient temperature range which can be set is 30ºC (96-well block) and the minimum
is 1ºC; the lowest temperature is 10ºC or 20ºC below ambient and the hottest temperature is 100ºC.
Examples of acceptable gradients for a 96-well block are given in the table below:
Lowest temperature
(ºC)
Set temperature
(ºC)
Highest temperature
(ºC)
Gradient
(ºC)
50 55 60 10
10 25 40 30
80 90 100 20
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
Set
temperature
Lowest
temperature
Highest
temperature
Gradient
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
Set
temperature
Lowest
temperature
Highest
temperature
Gradient