Multichannel Systems HiClamp Manual - Manual (Page 25)

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5 TEVC Recording Protocol
5.1 Preparations for Recording
Installing solutions
Solutions applied to the washing station are pumped by the peristaltic pumps. Verify that
the tubings are properly disposed and placed in the solution. Clicking the “Manual” icon
activates the manual command dialog which allows the manual control of the pumps.
Filling of all used tubings is a precondition for proper function of the washing station during
recording. Please verify that the liquids flow regularly without the appearance air bubbles
in the perfusion chamber.
The Manual Window
The manual window is designed for the manual control of the solution, for placing the basket
into the washing station, for unloading the oocyte and for the cleaning of the basket. A single
click on the valve buttons switches on the desired pump. When all pumps are turned off, the
radio button for the value 0 is highlighted in blue.
The sliders and values on the right indicate the rotation speed of the inlet peristaltic pumps.
Note that the rotation speed of the outlet suction pump cannot be adjusted and keeps on
running at optimum as long as the HiClamp is switched on.