Luminex Flock Monitor Quick Guide - Manual (Page 2)

89-30000-00-261 Revision
A - Page 2 of 2
Protect microspheres, conjugate, and detection antibody
from prolonged light exposure throughout entire procedure.
USA: 1-877-8-Biovet (824-6838)
Canada & Intl: 1-888-8-Biovet (824-6838)
Before You Start
Prepare reagents, samples and controls as described on page 1 of this Quick Guide. All steps below are performed
at room temperature, taking care to protect MICROSPHERES, SA-PE CONJUGATE, and DETECTION ANTIBODY
from light by wrapping in foil during incubation.
Plate Layout
Use this template for setting up your assay plate and
software batch on the Luminex 200 / FlexMAP 3D System.
WASH STEP - May be done with manual or automatic
washer technique
Allow plate to sit on magnet for 60 seconds
Remove liquid from wells
Add 100 μl WASH to each well
Repeat WASH STEP one more time (total of two
washes)
Resuspend MICROSPHERES in 100μl WASH with
a pipette or plate shaker
Read assay results on a Luminex analyzer
Shake plate at 800 rpm for 30 minutes
WASH
READ
IN
CUB
ATE
WASH STEP - May be done with manual or
automatic washer technique
Allow plate to sit on magnet for 60 seconds
Remove liquid from wells
Add 100 μl WASH to each well
Repeat WASH STEP one more time (total of two
washes)
Remove Final WASH from Wells
Add100 μl DETECTION ANTIBODY to each well
Cover plate with opaque lid or foil
Shake plate at 800 rpm for 30 minutes
WASH STEP - May be done with manual or
automatic washer technique
Allow plate to sit on magnet for 60 seconds
Remove liquid from wells
Add 100 μl WASH to each well
Repeat WASH STEP one more time (total of two
washes)
Remove final WASH from wells
Shake plate at 800 rpm for 60 minutes
Allow liquid components to equilibrate to room
temperature for 30 to 60 minutes*
Warm up and calibrate Luminex instrument*
Vortex working MICROSPHERES 5 seconds
Sonicate 5 seconds
Add 50 μl MICROSPHERES to each well
Vortex diluted CONTROLS and diluted SAMPLES
Add 50 μl CONTROLS or SAMPLE to
corresponding wells
Cover plate with opaque lid or foil
SET UP
WASH
IN
CUB
ATE
IN
CUB
ATE
WASH
DETECTION ANTIB
OD
Y
*Consult the product insert for more information
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
-
-
+
+
Add 100 μl SA-PE CONJUGATE to each well
Cover plate with opaque lid or foil
CONJUGA
TE
UNITED STATES
Luminex Corporation
Austin, Texas
Tel: +1.512.219.8020
Fax: +1.512.219.5195
CANADA
Luminex Molecular Diagnostics
Toronto, Ontario
Tel: +1.416.593.4323
Fax: +1.416.593.1066 EUROPE
Luminex B.V.
Oosterhout,The Netherlands
Tel: +31.162.408333
Fax: +31.162.408337
www.luminexcorp.com CHINA
Luminex Shanghai Trading Co..
Shanghai, China
Tel: +21.616.50809
Fax:+21.616.50811
[email protected]
JAPAN
Luminex Japan Corporation, Ltd.
Tokyo, Japan
Tel: +81.3.5545.7440
Fax:+81.3.5545.0451
AUSTRALIA
BSD Robotics, A Luminex Company
Brisbane, Queensland
Tel: +61.7.3273.0273
Fax:+61.7.3273.0274