Hach-Lange BODTRAK II: Respirometric Biochemical Oxygen Demand (BOD) - Manual (Page 24)

BODTrak™ II procedures
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5.4 Hach Standard Method procedure
Required apparatus:
Thermometer
BODTrak II bottle
Blender (optional)
Graduated cylinder
Tensette pipet and pipet tips
Wash water bottle
Required reagents:
1 nutrient buffer pillow
Additional nutrient or buffer (optional)
Deionized water
Before starting the test:
Use the sample volume table to choose the correct sample size ( Table 6 ).
If seeding samples, prepare a seed blank before preparing a sample. Treat the seed blank
the same as any other sample and omit step 5.
Refer to section 5.7 on page 33 for special considerations.
Table 6 Hach Standard Method sample volumes
BOD range
(mg/L)
Sample
volume (mL)
Seed volume
(mL)
Final volume
(mL)
Dilution
factor
0 to 35 370 10 to 35 420 1.14
0 to 70 305 10 to 35 355 1.16
0 to 350 110 10 to 35 160 1.45
0 to 700 45 10 to 35 95 2.11
Note: If seed strength is unknown, use 20 mL. Adjust seed volume as necessary to achieve
optimum results.