Activeforever CIDEX Plus Solution - Manual (Page 5)

mixture was dried on Petri dishes and recovered as
described above. Exposure to 0.5% CIDEX
® OPA Solution
for 5 minutes at 20°C caused a Ͼ4-log reduction in the
number of viable virus particles.
15 (See Table 3)
Table 3: Virucidal Test with CIDEX ® OPA Solution,
5 Minutes at 20°C (Pass Test= Ͼ3-log Reduction)
Virus Tested Result
Adenovirus Type 5 Pass test
Herpes Simplex Type 1 (HSV1) Pass test
HSV 2 Pass test
Influenza A (Hong Kong) Pass test
Coxsackievirus Type B3 Pass test
Polio Type 1 (Brunhilde strain) Pass test
Rhinovirus Type 37 Pass test
Vaccinia Pass test
HIV Type 1 Pass test
Cytomegalovirus Pass test
Human Coronavirus Pass test
Rotavirus (Strain WA) Pass test
Duck Hepatitis B Virus (surrogate for Hepatitis B) Pass test
Bovine viral diarrhea virus (surrogate for Hepatitis C) Pass test
Rotavirus (Strain WA) Pass test
Feline calicivrus as (surrogate for Norovirus) Pass test
Avian influenza A (H5N1) virus Pass test
Canine Parvovirus Pass test
Hepatitis A virus Pass test
Temperature
The effect of temperature on biocidal activity was further
assessed in quantitative tests of Bacillus subtilis spores
(ATCC #19659). Suspensions of spores were diluted
into various concentrations of CIDEX
® OPA Solution
and tested at 35°C, 40°C, 45°C, and 50°C. Results
showed that the solution is sporicidal and that, within
defined parameters, study temperature has a bigger
influence on the degree of sporicidal activity than the
concentration of the solution. For example, a у6-log
reduction in the number of viable spores occurred with
2-hour exposure to 0.05% disinfectant at 50°C, and a
3-log reduction was observed in the more concentrated
solution of 0.3% OPA at 35°C.
16
Organic Soil
Cleaning of instruments before disinfection is the
first step in removing bioburden from instruments.
However, manual cleaning is known to have variable
results,
25 potentially reducing the effectiveness of
disinfection. To assess the impact of an organic load
on bactericidal activity of 0.3% CIDEX
® OPA Solution,
suspensions of S. aureus and Pseudomonas aeruginosa
were exposed to 0.3% CIDEX OPA Solution at room
temperature (20°C) in the presence of 0%, 5%, 20%,
and 40% horse serum. For exposure times of up to
5 minutes, bactericidal activity decreased with increasing
concentration of horse serum. However, when exposure
to 0.3% CIDEX OPA Solution was 10 minutes or longer,
these concentrations of horse serum did not prevent
Ͼ7-log reduction of viable bacteria.
17
Figure 2. Biocidal Activity of 0.3% CIDEX ® OPA Solution.
Biocidal activity of 0.3% CIDEX ® OPA Solution was evaluated in
the presence of variable amounts of horse serum. Suspensions of
Staphyloccoccus aureus (SA) or Pseudomonas aeruginosa (PA) were
diluted 1:10 into 0.3% OPA+horse serum. After exposure to disinfectant,
viable organisms were assayed quantitatively.17
Summary
For more than 10 years, CIDEX ® OPA Solution has
been successfully utilized by healthcare facilities
worldwide to high-level disinfect reusable medical and
dental devices. As part of its commitment to infection
prevention, ASP has conducted extensive tests to
evaluate the efficacy of CIDEX OPA Solution. Results
show that CIDEX OPA Solution is bactericidal—including
for MRSA and VRE—mycobactericidal, sporicidal, and
virucidal—including for surrogates for HBV and HCV.
CIDEX ® OPA Solution is currently used to disinfect a
wide variety of devices, including delicate and costly
flexible gastroenterology endoscopes. This range of
applications is possible because of the solution’s broad
materials compatibility. An important benefit of broad
materials compatibility is simple standardization of
processing protocols—a single protocol can be
developed for disinfection of many items.
The fast processing time and ease of use of CIDEX ® OPA
Solution also contribute to rapid turnaround time,
allowing smaller inventories of instruments and
potential cost savings. Cost savings also might be
realized by eliminating the monitoring of air and
personnel exposure required with glutaraldehyde.
Combined, these factors demonstrate why CIDEX ® OPA
Solution makes both clinical and economic sense for
facilities seeking an effective, more cost-efficient
option for instrument reprocessing. Furthermore,
the efficacy testing and quality assurance measures
applied by ASP should be an important consideration
for healthcare personnel as they work toward the
control and prevention of HAIs.
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Exposure Time (minutes)
SA vs OPA + 5% serum
PA vs OPA + 5% serum
SA vs OPA + 20% serum
PA vs OPA + 20% serum
SA vs OPA + 40% serum
PA vs OPA + 40% serum
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