Iris Sample Processing StatSpin® Express 3 Primary Tube Centrifuge - Manual (Page 10)

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2. Slide the rotor onto the shaft and push down gently to seat the drive pins in the holes.
3. Place the retaining nut on the shaft and tighten securely using the 5/16 inch hex key.
You will not be able to tighten the retaining nut if the drive pins are not properly aligned. If this
occurs lift the rotor off and repeat the process until it is seated.
Spinning the Sample
CAUTION - Never operate the Express 3 without the rotor locked securely in place. Failure to
properly install and secure the rotor may result in damage to the centrifuge not covered by warranty.
Loading
1. Ensure the appropriate tube inserts are installed. The Express 3 centrifuge is provided with
eight tube inserts for 13 x 75 mm tubes (Product No. SV08) and eight tube inserts for 16 x 100
mm tubes (Product No. SV07). Other sizes are available (See accessories).
2. The rotor must be properly balanced to ensure smooth operation. DO NOT spin a single tube.
3. Use the same size and style tubes in opposite positions. Balance liquid in tubes to within 0.5 mL.
CAUTION
- Replace Tube Insert immediately if a tube breaks during centrifugation.
CAUTION - Replace tubes used for rotor balancing after 10 spin cycles to reduce chances of
breakage due to the repeated stress of centrifugation.
4. Close and latch the centrifuge cover.
5. Select the spin time by pressing the appropriate cycle time button.
6. Press Start button.
Cycle Settings
The operator should select spin time settings to achieve optimum results for specific applications.
The following are general guidelines:
Setting Speed RCF
2 min Produces high quality plasma/serum from whole
blood for chemistry, coagulation and cardiac
testing.
7200 rpm 4440 xg
3 min Produces high quality plasma/serum in gel tubes
and large plastic collection tubes from whole blood
for chemistry or coagulation testing.
7200 rpm 4440 xg
5 min Produces separation equivalent to 3 min setting.
Centrifugal forces are decreased to accommodate.
larger glass tubes which require lower g-forces.
5600 rpm 2685 xg
Tube Guide by Settings
Tube
Size Insert Tube
Dimension
2 minute 3 minute 5 minute
5 mL SV08 13x75 mm Plastic Plastic/Gel Glass/Plastic/Gel
7 mL SV06 16x75 mm Plastic Plastic/Gel Glass/Plastic/Gel
7 mL SV09 13x100 mm Plastic Plastic/Gel Glass/Plastic/Gel
10 mL SV07 16x100 mm Plastic Plastic/Gel Glass/Plastic/Gel