Bio-Rad Bio-Plex Pro™ Wash Stations - Manual (Page 51)

Defining New Programs
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 51
Drip Mode
The slowest dispensing mode is the drip mode. This mode is not recommended for
xMap or Bio-Plex applications.
Drip mode is used for very sensitive applications, e.g. washing cell layers, because
the instrument offers the possibility to dispense liquid in small drops. This minimizes
the detachment of cells and increases the remaining cell percentage at the bottom of
the wells.
The following example shows a typical wash program for working with adherent cells
as it would appear in the Define/Edit menu. See Define/Edit a Program on page 49
for further information about defining programs.
Note
The following example program should not be used as a standard wash
procedure for cell washing as it is necessary to adjust the wash
parameters, such as Z-position “MOVE CUST.”, dispensing and aspirating
rates, head speed, etc., according to the cell type used.