Bio-Rad Bio-Plex Pro™ Wash Stations - Manual (Page 50)

Defining New Programs
50 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Aspirating Modes
To improve wash efficiency and reduce the residual volume, the manifold must be
positioned so that the aspirating needles are correctly positioned in the wells for
round-bottom, V-shaped bottom, or flat-bottom well microplates.
Normal AspirationMode
For round-bottom or V-shaped bottom wells, the aspirating needles are placed in the
middle of the wells. Only one aspiration position can be selected.
Round Bottom Wells
'V' Shaped Wells
Normal Aspirating Positions
Crosswise Aspiration Mode
With flat bottom wells, the instrument can perform crosswise aspiration using two
aspiration positions per well.
The aspirating needles are set at two positions on the bottom of the wells
(front edge and back edge).
Aspirate Y Offset 1 Aspirate Y Offset 2
Front edge Back edge
Crosswise Aspiration for Flat Bottom Wells