Luminex 100 User Manual Version 1.7 - Manual (Page 126)

A - 4 PN 89-00002-00-063 Rev. A
Luminex 100 User Manual Version 1.7 x MAP Technology
xMAP microsphere set Luminex multi-analyte microspheres containing a unique mixture of
two distinctly-colored fluorochromes to distinguish them from other
multi-analyte microspheres.
laser Light Amplification by Stimulated Emission of Radiation (laser).
This highly purified source of light is an efficient way to excite
fluorochrome electrons.
LumAvidin
microspheres An xMAP microsphere set that has been chemically modified so that
its surfaces are covered with avidin.
MAP Multiple Analyte Profile.
microparticle A solid substance with a diameter in the micrometer range. Often
used as a synonym for an xMAP microsphere.
microspheres Polystyrene spheres with a diameter in the micrometer range. Also
called beads.
multi-analyte Several assays or tests performed simultaneously in the same
reaction container.
multi-analyte panel A particular group of tests or assays performed simultaneously on
distinct xMAP microsphere sets.
particle In this application, used to describe a microsphere.
PE See Phycoerythrin.
photobleaching The process in which light absorption converts the fluorochromes
inside the beads to different fluorescent or nonfluorescent
compounds. Photobleaching prevents beads from being properly
classified.
PMT Photomultiplier tube, which measures the excitation emission.
Phycoerythrin (PE) A popular red fluorochrome commonly used as a reporter. Excitation
= 546 nm, Emission = 575 nm.
quenching Process by which a fluorochrome is affected by itself or another
fluorochrome in the same solution because of the close proximity of
the excitation and emission peaks.
region A designated area specific to a particular bead ID.