Hoefer HE-PLUS System - Manual (Page 22)

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Solutions
Tris Acetate EDTA Buffer (TAE)
1X Working Concentration:
40 mM Tris base
20 mM Glacial Acetic Acid (NaOAc)
2.0 mM EDTA
pH 8.3
10X Stock Solution:
48.4 g Tris Base
16.4 g or 11.42 ml NaOAc
7.4 g EDTA or 20 ml 0.5 M EDTA (pH 8.0)
H
2
O to 1 liter
Tris Borate EDTA Buffer (TBE)
1X Working Concentration:
89 mM Tris Base
89 mM Boric Acid
2.0 mM EDTA
pH 8.0
10X Stock Solution:
108 g
Tris Base
55 g Boric Acid
6.72 g EDTA or 40 ml 0.5 M EDTA (pH 8.0)
H
2
O to 1 liter
Sample Loading Buffer, DNA
6X Stock Solution:
30%
Glycerol in H
2
O
0.25% Xylene cyanol
0.25% Bromophenol blue
pH 8