Hoefer HE-PLUS System - Manual (Page 12)

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p2
1
Make 500 ml of either 1X TAE or 1X TBE
electrophoresis buffer.
2
Weigh an appropriate quantity of agarose (see
Table 1) and place it into a 250 ml flask. Add a
sufficient quantity of either 1X TAE or 1X TBE buffer
(prepared in step 1) to achieve a final volume of
100 ml agarose solution.
Table 1: Gel Concentrations and Resolving Ranges
Concentration Agarose (g) Efficient Range
of Agarose in per 100 ml of Separation
Gel (%w/V) Buffer of Linear DNA (Kb)
0.3 0.3 5 – 60
0.6 0.6 1 – 20
0.7 0.7 0.8 – 10
0.9 0.9 0.5 – 7
1.2 1.2 0.4 – 6
1.5 1.5 0.2 – 3
2.0 2.0 0.1 – 2
Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6.8 613.