Physitemp Instruments BFS-TC - Manual (Page 4)

4.0 TYPICAL STAGE PERFORMANCE
The degree of cold obtained is dependent on the temperature of the cooling water. The stage temperature
should reach 55-65° below that of the cooling water. Therefore, a stage temperature of -40° can be
achieved under normal conditions. 1 1/2” thickness of liver which covers the stage should freeze within
15-20 minutes. Temperature throughout the tissue should drop to -20°C in a further ten minutes. Tissue
under 1/16” in thickness should be ready for sectioning within 90 seconds after switching on.
5.0 MEASUREMENT AND CONTROL OF TISSUE TEMPERATURE
5.1 One of the main advantages of these freezing stages is the ease with which the temperature of
frozen tissue can be changed. Optimum cutting temperature varies for different tissue and is best deter-
mined by experiment. the following temperatures have been found satisfactory:
TISSUE TEMPERATURE °C
Kidney -15
Brain -18
Liver -18
Thyroid -20
Skin -30
Keloid -35
Patella -40
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