ChemoMetec NC-200 User Manual
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How to Perform Total Cell Count
and Viability of Aggregated Cells
Select the protocol from the menu
‘Viability and Cell Count - Aggregated Cells Assay’
Optional:
Enter Sample ID and Operator Name
Add equal volumes of ‘Solution 10’ and your cell sample to a
microcentrifuge tube and mix. Retain an aliquot of at least 200 μL
cell suspension without ‘Solution 10’ for analysis in step 5.
Immediately load your sample into a Via1-Cassette
™
and press ‘Run’
After analysis, follow the on-screen prompt and load a sample
of your cells NOT treated with ‘Solution 10’ and press ‘Run’.
The compensation for the sample dilution with ‘Solutions 10’ is automatically per-
formed by the software. However, if the proportions are different from those above please
add the correct volumes in the input fields under the image window prior to running your
assay. The entered volumes are used for calculating the cell concentration in the original
cell sample.
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